Table 5.
Endogenous 20-HETE and ω-hydroxylase activity in control and hyperandrogenemic female (DHT-treated) SS.5Bn and CYP4A2−/− rats
| Endogenous 20-HETE, pmol/mg |
ω-Hydroxylase Activity pmol 20-HETE·min−1·mg−1 |
|||
|---|---|---|---|---|
| Cerebral vessels | Renal vessels | Cerebral vessels | Renal vessels | |
| SS.5Bn female rats | ||||
| Control | 0.047 ± 0.010 (n = 3) | 0.059 ± 0.006 (n = 3) | 0.034 ± 0.004 (n = 6) | 7.346 ± 0.588 (n = 5) |
| DHT | 0.082 ± 0.019 (n = 3) | 0.118 ± 0.009 (n = 3) | 0.033 ± 0.004 (n = 8) | 3.715 ± 0.330 (n = 7) |
| P value | NS | <0.005 | NS | <0.01 |
| CYP4A−/− female rats | ||||
| Control | 0.029 ± 0.004 (n = 5) | 0.035 ± 0.008 (n = 5) | 0.023 ± 0.004 (n = 8) | 4.299 ± 0.8760 (n = 8) |
| DHT | 0.056 ± 0.006 (n = 4) | 0.075 ± 0.026 (n = 3) | 0.068 ± 0.016 (n = 7) | 13.789 ± 0.9841 (n = 7) |
| P value | <0.01 | NS | <0.001 | <0.0001 |
Statistics are from two-way ANOVA comparison of DHT- versus placebo-treated control rats. Statistics for intergroup comparison are in text.