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. 2016 May 4;311(1):F120–F130. doi: 10.1152/ajprenal.00456.2015

Fig. 10.

Fig. 10.

Absence of albumin decreased tubular cell apoptosis in DKO kidneys. TUNEL staining (green) identified increased numbers of apoptotic cells in Col4a3−/−;Alb+/− kidneys (A) compared with DKO kidneys (C). In control kidneys (B), there were only occasional (<1 cell/high-power field) apoptotic cells within the interstitium. Counterstaining with anti-nidogen (red) labeled all basement membranes. Original magnification was ×200. Scale bar = 50 μm. D: quantification of tubular cell apoptosis as measured by the number of TUNEL-positive nuclei per 100 tubules. The difference between Col4a3−/−;Alb+/− kidneys and kidneys from DKO and control mice was significant (*P = 0.022 and 0.016, respectively). There were significantly more apoptotic tubular cells in DKO kidneys compared with control kidneys (*P = 0.016).