Fig. 7.

HDE-induced ICAM-1-specific immunostaining on airway luminal epithelial cells is abrogated when mice are pretreated with salbutamol. Paraffin-embedded lung sections from mice exposed a single time to 12.5% HDE (B) demonstrate prominent epithelial ICAM-1 immunostaining compared with mice given saline alone (A). Tissues from mice pretreated with nebulized salbutamol and then challenged with HDE showed a marked reduction in ICAM-1 staining (C). Micrographs are representative of lung sections from 3 mice per condition. Arrows indicate localization of ICAM-1 on the apical surface of the epithelium. Parenchymal alveolar cells stained equally positive in all groups.