Fig. 11.

Immunofluorescence confirms the presence of GFP⁺ recruited macrophages in the alveoli. Frozen lung sections from hCD68rtTA-GFP mice day 6 after LPS, fed doxycycline for 9 days, were cut and stained with tomato lectin (grey), anti-murine CD68 (red), and DAPI (blue). Endogenous hCD68-rtTA GFP reporter expression (green) was preserved through freezing. A and B: representative photos showing GFP⁺ Mø in alveoli. A: isotype control. B: anti-murine CD68. Pink arrows: CD68⁺ Mø that do not express GFP. Green arrows: CD68⁺ Mø that express GFP.