Skip to main content
. 2016 May 17;311(1):L87–L100. doi: 10.1152/ajplung.00141.2016

Fig. 14.

Fig. 14.

Immunofluorescence confirms that tissue mononuclear phagocytes express hCD68-rtTA Cre-driven TdTomato reporter while resAMø do not. Frozen lung sections from naïve hCD68rtTA-TdTomato mice fed doxycycline for 12 days were cut and stained with tomato lectin (grey), anti-murine CD68 (green), and DAPI (blue). Endogenous hCD68-rtTA TdTomato reporter expression (red) was preserved through freezing. A and B: representative photos of alveoli containing resAMø but not TdTomato-reporter+ cells. A: isotype control. B: anti-murine CD68. C and D: representative photos around large airways containing GFP-reporter+ cells within the lung tissue. C: isotype control. D: anti-murine CD68. Z-stack of the area of interest shows anti-murine CD68 staining colocalizes with hCD68rtTA-TdTomato, inset in white box. Pink arrows: ResAMø. White arrows: tissue CD68+ cells that do not express TdTomato. Green arrows: tissue CD68+ cells that express TdTomato.