Fig. 3.
hCD68rtTA-GFP resAMø do not activate GFP even when doxycycline is provided ex vivo. ResAMø and resPMø were isolated by lavage from hCD68rtTA-GFP mice and cultured on glass cover slips in the presence or absence of 20 μg/ml doxycycline for 4 days. Cells were fixed and mounted with DAPI (blue). Tet-On GFP (green) expression was assessed by microscopy. A: resAMø cultured without doxycycline. B: resAMø cultured with doxycycline. C: resPMø cultured without doxycycline. D: resPMø cultured with doxycycline.