Fig. 4.

Flow cytometry gating strategy for lung tissue cells. Flow cytometry was used to identify cells collected from lung digest. Cells of interest were identified by the following gating strategy: higher SSC cells were selected, doublets were excluded, CD45⁺ cells were selected, Ly6G⁺ neutrophils were excluded, and high SSC F4/80⁺ cells were selected. Mø subpopulations were separated into tissue Mø (CD11c^−/lowCD11b⁺) and resAMø (CD11c⁺CD11b^low). DC were identified within SSC^lowF4/80^mid/− cells as Ly6C⁻CD11c⁺ MHCII^high. Subpopulations of DC were separated into CD11b⁺DC (CD11b⁺CD103⁻) and CD103⁺DC (CD11b⁻CD103⁺).