Fig. 8.

Flow cytometry gating strategy for peripheral blood. Flow cytometry was used to identify cells collected from lysed peripheral blood. Residual RBC and platelets were excluded by gating out low FSC events, CD45⁺ cells were selected, and Ly6G expression was used to identify neutrophils and eosinophils as the two high SSC populations (eosinophil identity could be confirmed by SiglecF expression, data not shown). Monocytes were identified within Ly6G⁻SSC^low cells as CD11b⁺CD115⁺, while remaining cells were lymphocytes.