Fig. 5.
ASIC1 Ca2+ influx contributes to ET-1-induced NFATc3 nuclear import. A: mouse PASMC from ASIC1+/+ or ASIC1−/− mice were transfected with NFATc3-enhanced green fluorescent protein (EGFP). EGFP nuclear (Fn)/cytosolic fluorescence (Fc) was determined in PASMC treated with vehicle, ET-1 (100 nM, B), or ionomycin (0.1 μM, C). PASMC from ASIC1+/+ mice were additionally treated with PcTX1 (20 nM, A and B). Values are means ± SE, n = 3 replicates/group from average of 20–25 cells/each replicate. *P < 0.05 vs. vehicle, #P < 0.05 vs. ASIC1+/+ mice analyzed by a two-way ANOVA and individual groups compared with the Student-Newman-Keuls test.