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. 2016 May 17;311(1):L48–L58. doi: 10.1152/ajplung.00040.2016

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Fig. 6. — Knockout of ASIC1 does not alter NFAT, Orai1, or TRPC1 mRNA and protein levels in PASMC. Fold change (from calibrator) in NFATc2 (A), NFATc3 (B), Orai1 (C), and TRPC1 (D) mRNA expression in PASMC from ASIC1^+/+ and ASIC1^−/− mice. β-Actin was used as the endogenous control. Also shown are representative Western blots (top) and summary data (bottom) showing Orai1 (E) and TRPC1 (F) protein expression in PASMC from ASIC1^+/+ and ASIC1^−/− mice. GAPDH was used as the endogenous control. Values are means ± SE, n = 6 from different passages and at least two different mice, analyzed by unpaired t-test.