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. 2016 May 17;311(1):L48–L58. doi: 10.1152/ajplung.00040.2016

Fig. 7.

Fig. 7.

Colocalization of ASIC1, PICK1, and calcineurin B in mouse PASMC. Representative confocal images of the Duolink PLA interaction (red puncta) between ASIC1-PICK1 (left), ASIC1-calcineurin B (middle), and PICK1-calcineurin B (right) in PASMC from ASIC1+/+ mice (A) and ASIC1−/− mice (B). Proximity Ligation Assay (PLA) interactions are not detected for ASIC1-PICK1 or ASIC1-calcineurin B in PASMC from ASIC1−/− mice. For negative controls (C), PASMC were incubated with each primary alone and both PLA probes. Actin is labeled with Alexa Fluor 647 phalloidin (blue) and the nuclei are labeled with SYTOX (green).