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α- and β-Myosin heavy chain (MyHC) isoform content. Several dilutions of the same sample are used to capture both α- and β-MyHC in the linear densiometric range. Skeletal soleus muscle was used as a standard to visualize separation of α (band on top)- and β (band on bottom)-isoforms. At 2 mo, WT or HCM mice of either sex display no significant expression of β-MyHC (<2%).
