Fig. 7.
Western blot analysis of phospho (p)-cardiac troponin I (cTnI)-Ser23/24 and p-myosin-binding protein (cMyBP)-C-Ser282. Left ventricular myocardial samples from male, female, WT, or HCM mice (n = 4/group) were separated by SDS-PAGE. A: Western blot analysis and bar graph summary of p-cTnI-Ser23/24; phosphoprotein was normalized to total protein. B: Western blot analysis and bar graph summary of p-cMyBP-C-Ser282; phosphoprotein was normalized to total protein. Two-way ANOVA identified significant sex effect for p-cTnI-Ser23/24 but no difference for p-cMyBP-C-Ser282. *P < 0.05 from WT counterpart.