Table 3.
Summary of disease- or sex-specific effects
| Factor | Sex | HCM | Sex × HCM | Post Hoc |
|---|---|---|---|---|
| Ca2+ sensitivity | * | − | − | ↓M-HCM |
| ktr | − | * | − | ↑M-HCM |
| Tension cost | − | − | * | ↑M-HCM |
| Cardiac remodeling | ||||
| Collagen, % | − | − | − | − |
| HW/TL | * | * | * | ↑M-HCM |
| EF, % | − | − | * | ↑F-HCM |
| RWTd | − | − | − | − |
| ProQ Diamond | ||||
| cMyBP-C | * | * | − | − |
| 97 kDa | − | * | − | ↑F/M-HCM |
| Desmin | − | − | * | − |
| cTnT | − | − | * | ↑F-HCM |
| cTnI | − | * | * | ↑F-HCM |
| Western blot | ||||
| p-cTnI-Ser23/24 | * | − | − | ↓M-HCM |
| p-cMyBP-C-Ser282 | − | − | − | − |
Values are means ± SE; n, no. of subjects. EF, ejection fraction; RWTd, relative wall thickness during diastole; cMyBP-C, cardiac myosin-binding protein C; cTnT, cardiac troponin T; cTnI, cardiac troponin I, p, phospho. This summarizes the observed effects of sex and the HCM transgene in WT, HCM, male, and female mice. Using a 2-way ANOVA followed by Bonferroni's post hoc test to compare differences between groups, we designate a significant
*
(P < 0.05) effect or no change (−) with each factor. In addition, post hoc analysis determined whether HCM increased (↑) or decreased (↓) the parameter with respect to sex.