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. 2016 May 6;37(8):799–809. doi: 10.1093/carcin/bgw059

Figure 5.

Figure 5.

Cdk1 is involved in Cdc6 stability and polyploidy formation in E7-expressing cells. E7-expressing cells were transfected with 20nM si-Cdk1 or control siRNAs for 72h. (A) Downregulation of Cdk1 increased Cdc6 expression in E7-expressing RPE1 cells (left) or NIKS (right). Cdc6 levels were determined using immunoblotting. (B) Downregulation of Cdk1 induced polyploidy formation in E7-expressing NIKS. The cell cycle profile was analyzed using flow cytometry. The data from a representative experiment of two independent experiments are shown. (C) The Cdk1 inhibitor RO-3306 increased the Cdc6 level and efficiently induced polyploidy formation in RPE1 E7-expressing cells. RPE1 cells expressing E7 or vector were treated with 7.5 µM RO-3306 or dimethyl sulfoxide (DMSO) for 48h. Cdc6 levels were determined using immunoblotting, and the cell cycle profile was analyzed using flow cytometry. The data from at least three independent experiments are shown. NS-siRNA, non-silencing siRNA.