Table 2.

Top 20 ranked E3 ubiquitin ligases based on Bayesian analysis

Column 1 Rank	Column 2 Gene Symbol	Column 3 Class	Column 4 Initial Probability	Column 5 Rat RNA-seq	Column 6 mpkCCD Transcriptome	Column 7 mpkCCD Proteome	Column 8 Dot Product w/AQP2	Column 9 UniProt Subcellular Location	Column 10 UniProt Domain/IMP
1	Nedd4	HECTc	0.0027	0.0058	0.0104	0.0122	0.0171	0.0198	0.0271
1	Nedd4L	HECTc	0.0027	0.0058	0.0104	0.0122	0.0171	0.0198	0.0271
3	Amfr	RING	0.0027	0.0058	0.0104	0.0122	0.0146	0.0170	0.0232
4	Stub1	UBOX	0.0027	0.0058	0.0104	0.0122	0.0220	0.0255	0.0217
5	Itch	HECTc	0.0027	0.0043	0.0078	0.0092	0.0128	0.0149	0.0203
6	Zfpl1	RING	0.0027	0.0058	0.0076	0.0090	0.0126	0.0146	0.0199
7	Trip12	HECTc	0.0027	0.0058	0.0104	0.0122	0.0171	0.0198	0.0169
8	Prpf19	UBOX	0.0027	0.0043	0.0078	0.0092	0.0165	0.0191	0.0163
9	Syvn1	RING	0.0027	0.0043	0.0057	0.0068	0.0094	0.0109	0.0150
10	Anapc11	RING	0.0027	0.0058	0.0104	0.0122	0.0146	0.0170	0.0145
11	March5	RING	0.0027	0.0058	0.0055	0.0064	0.0090	0.0104	0.0142
12	Rnf130	RING	0.0027	0.0029	0.0038	0.0045	0.0081	0.0094	0.0128
13	Ppil2	UBOX	0.0027	0.0043	0.0078	0.0092	0.0128	0.0149	0.0127
14	Brap	RING	0.0027	0.0058	0.0076	0.0090	0.0126	0.0146	0.0125
15	Smurf2	HECTc	0.0027	0.0043	0.0078	0.0092	0.0073	0.0085	0.0116
16	Trim25	RING	0.0027	0.0029	0.0052	0.0061	0.0110	0.0127	0.0109
17	Cyhr1	RING	0.0027	0.0058	0.0076	0.0090	0.0108	0.0125	0.0107
18	Rnf128	RING	0.0027	0.0058	0.0104	0.0064	0.0064	0.0074	0.0102
18	Rnf167	RING	0.0027	0.0058	0.0104	0.0064	0.0064	0.0074	0.0102
18	Rnf5	RING	0.0027	0.0058	0.0104	0.0064	0.0064	0.0074	0.0102

The 20 E3 ubiquitin ligases most likely to ubiquitinate aquaporin-2 (Aqp2) based on Bayesian analysis. The Bayesian analysis is sequential, with column 10 being the final ranking of E3 ligases. Columns 5–10 show the Bayesian probabilities for the E3 ligases after including the following datasets: rat RNA-seq transcriptomic data in collecting duct tubule segments (column 5) (15), mpkCCD transcriptomic data from Affymetrix expression microarray experiments (column 6) (33), mpkCCD proteomic data from LC-MS/MS quantification (column 7) (32), the dot product of each E3 ligase and Aqp2 in subcellular fractionation data (column 8) (32), the location of the E3 ligase within the cell based on UniProt Gene Ontology cellular component terms (column 9), and E3 ligases identified in UniProt as integral membrane proteins or having membrane-interacting domains (column 10).