Table 2.
Identified bacteria with molecular methods in periprosthetic tissue and sonicate fluid among the patients with PJI
| A | B | C | D | E | F | G | H | I | J | K | L | M | N | O |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 86 | 25 | M | K | + | − | + | + | + | + | − | S. aureus | 2 | S. aureus |
| 2 | 79 | 39 | M | H | − | − | + | − | − | + | + | S. aureus | 6 | S. aureus |
| 3 | 60 | 11 | F | H | + | − | − | − | + | − | − | / | 0 | P. avidum |
| S. aureus | 5 | S. aureus | ||||||||||||
| S. epidermidis | 5 | S. epidermidis | ||||||||||||
| 4 | 83 | 66 | M | H | + | − | − | − | − | − | + | Streptococcus sp | 4 | Streptococcus sp |
| 7 | 87 | 89 | F | K | + | − | − | − | + | + | + | Staphylococcus sp | 6 | Staphylococcus sp |
| 12 | 73 | 29 | M | H | + | − | − | − | + | + | − | S. mitis/oralis | 2 | S. mitis/oralis |
| 13 | 65 | 1 | M | K | − | − | − | − | − | + | + | S. aureus | 5 | S. aureus |
| 15 | 78 | 2 | M | H | + | + | − | − | − | − | + | / | 0 | S. epidermidis |
| E. faecalis | 6 | |||||||||||||
| 18 | 70 | 64 | F | H | + | − | − | − | + | + | + | S. epidermidis | 1 | E. faecalis |
| 21 | 70 | 1 | M | H | + | − | + | + | − | − | − | / | 0 | S. epidermidis |
| 22 | 70 | 100 | M | H | + | − | + | + | + | − | − | S. epidermidis | 3 | S. epidermidis |
| 23 | 59 | 80 | M | H | + | − | − | − | + | − | − | / | 0 | Parvimonas micra/ Micromonas micros |
| Staphylococcus sp | 4 | |||||||||||||
| S. aureus | 2 | |||||||||||||
| 24 | 66 | 12 | F | K | + | + | + | + | + | − | − | S. epidermidis | 2 | S. epidermidis |
| 29 | 73 | 189 | F | H | − | − | + | + | + | + | − | S. aureus | 6 | S. aureus |
| 35 | 66 | 152 | F | H | + | − | + | + | + | + | − | E. coli | 6 | E. coli |
| 37 | 61 | 0 | M | H | + | − | − | − | − | − | − | / | 0 | S. aureus |
| 40 | 74 | 46 | M | K | + | − | − | − | − | + | − | S. epidermidis | 2 | S. epidermidis |
| 44 | 83 | 36 | M | H | + | − | − | − | − | + | − | S. aureus | 3 | S. aureus |
| 47a | 54 | 60 | F | H | − | − | − | − | − | − | − | / | / | |
| 50 | 56 | 6 | F | K | + | − | + | + | + | − | + | S. sanguinis | 2 | S. sanguinis |
| 51 | 69 | 1 | M | K | + | − | − | − | + | − | − | Staphlyococcus sp | 3 | / |
| 52 | 69 | 118 | F | K | + | − | − | − | + | − | + | E. faecalis | 4 | Polymicrobialy |
| electroferogram | ||||||||||||||
| 53 | 78 | 51 | M | H | − | − | + | − | − | + | − | S. aureus | 3 | S. aureus |
| 64 | 74 | 104 | M | K | + | − | − | − | + | + | + | E. cloacae | 4 | E. cloacae |
| 67 | 71 | 8 | M | H | + | − | − | − | − | − | − | E. faecium | 3 | E. faecium |
| 68 | 88 | 213 | F | K | + | − | − | − | − | + | − | S. pasteuri | 3 | S. pasteuri |
| 73 | 63 | 23 | F | K | + | − | − | − | + | − | − | / | 0 | S. epidermidis |
| 77 | 74 | 2 | F | K | + | − | − | − | − | − | − | K. oxytoca/ | 3 | K. oxytoca/ |
| K. michiganensis | K. michiganensis | |||||||||||||
| 82 | 65 | 12 | M | H | − | − | + | + | + | − | − | K. oxytoca/ | 3 | K. oxytoca/ |
| K. michiganensis | K. michiganensis |
The patient was diagnosed with PJI based on culture results of periprosthetic tissue.
A Patient number
B Age of patient
C Time of implant in vivo, months.
D Sex
E Joint: H – hip, K – knee.
F C-reactive protein >10 mg/L.
G Erythrocyte sedimentation rate >30 mm/h.
H White blood cell count in synovial fluid >1.7 × 109/L.
I Polymorphonuclear neutrophil (PMN) count in synovial fluid >65%.
J Histology: > 5 polymorphonuclear neutrophils/high-power field.
K Purulence
L Fistula/sinus
M Identified bacteria in periprosthetic tissue.
N Number of positive samples with the same bacterial species identified with molecular methods.
O Identified bacteria by sonication.