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. 2016 Jul 30;17:17. doi: 10.1186/s12858-016-0073-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cusabio and Elabscience SIRPA ELISA kits failed to recognize recombinant human SIRPA protein. a Cusabio SIRPA ELISA kit (CSB-EL021334HU) recognized its own calibrator diluted in buffer from Cusabio ELISA kit generating a linear curve (diamonds) but did not recognize recombinant human SIRPA protein (squares); b Elabscience SIRPA ELISA kit (E-EL-H1573) recognized its own calibrator diluted in buffer from Elabscience ELISA kit generating a linear curve (diamonds) but did not recognize recombinant human SIRPA protein (squares). X- axis represents known standard concentration (ng/ml) and Y-axis represents optical density (OD) measured at 450 nm. c Western blot of rhSIRPA using SIRPA antibody (Biolegend, catalog # 323805) detected a ~55 kda band in rhSIRPA but not in calibrators from Cusabio and Elabscience SIRPA ELISA kits