Fig. 2.
Alveolar-capillary barrier disruption and increased permeability pulmonary are ameliorated in αDβ2-deficient mice infected with Plasmodium berghei ANKA. Infected wild type (α+/+D) and αDβ2-deficient (α−/−D) were study 7 days after infection. Bronchoalveolar lavage fluid (BALF) was performed. a Total protein concentration was measured in BALF samples using a BCA protein determination assay. b A second group of animals was sacrificed without BALF and the lungs were removed and weighed immediately after sacrifice. c In a third group of α+/+D and α−/−D mice, Evans Blue Dye (2 %, 0.2 mL) was injected intravenously. The animals were sacrificed 1 h later, and Evans Blue Dye concentration in the lung tissue. Each bar in panels a–c indicates the mean ± SEM. of determinations from 5 animals.#P ≤ 0.05 compared to the respective control group; P ≤ 0.05 compared to α+/+D infected mice