Fig. 4.
F4/80-positive leukocytes accumulate in the alveoli in Plasmodium berghei ANKA-induced experimental MA-ARDS and are reduced in αDβ2-deficient mice. Lung tissue from α+/+D and α−/−D mice infected with P. berghei was harvested at 7 days after infection. Lung slices were incubated with anti-F4/80 as the primary antibody and HRP-conjugated secondary antibody. The sections were examined and photographed using an Olympus BX41 microscope at ×200 magnification (scale bars 200 mm). a Sections from a control α+/+D mouse. b Section from a control α−/−D mouse. c Lung tissue from an infected α+/+D wild type animal. d Section from an infected α−/−D mouse. The staining patterns shown in a–d are representative of those in lung tissue from 3 individual mice for each condition