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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Xenobiotica. 2015 Sep 14;46(5):406–415. doi: 10.3109/00498254.2015.1086038

Table 3.

Enzyme kinetic parameters determined for 7-OH-glucuronidation of genistein, daidzein, and equol using pooled liver microsomes from dog (n=16), cat (n=5), and human (n=48).

Genistein
Species Vmax
(nmoles/min/mg protein)		 Km
(µM)		 Vmax/Km
(nmoles/min/mg/µM)
Dog 5.13 ± 0.2 15.3 ± 2.4 0.335 ± 0.07
Cat 2.15 ± 0.1 10.3 ± 2.5 0.209 ± 0.07
Human 1.60 ± 0.07 11.7 ± 2.4 0.137 ± 0.06
Daidzein
Species Vmax
(nmoles/min/mg protein)		 Km
(µM)		 Vmax/Km
(nmoles/min/mg/µM)
Dog 8.10 ± 0.1 55.4 ± 3.8 0.146 ± 0.02
Cat 1.61 ± 0.06 41.9 ± 6.6 0.038 ± 0.04
Human 1.30 ± 0.05 26.6 ± 5.1 0.049 ± 0.04
Equol
Species Vmax
(nmoles/min/mg protein)		 Km
(µM)		 Vmax/Km
(nmoles/min/mg/µM)
Dog 14.3 ± 0.6 32.6 ± 6.9 0.439 ± 0.09
Cat 20.4 ± 1.4 132 ± 26 0.155 ± 0.08
Human 17.4 ± 0.7 31.8 ± 5.1 0.547 ± 0.08