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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Tuberculosis (Edinb). 2016 Apr 29;99:70–80. doi: 10.1016/j.tube.2016.04.010

Figure 4. Validation of the MT1342-MT1352 operon.

Figure 4

(A) Genetic locus of the predicted operon that spans MT1342-MT1352. Numbered bold lines indicate IGRs that are PCR amplified. The lengths of IGRs are IGR1:-4nt, IGR2:-8nt, IGR3: 48nt. IGR4:29nt, IGR5: 6nt, IGR6: 44nt, IGR7:6nt, IGR8:39nt, IGR9:13nt and IGR10:7nt. Negative values indicate an overlap between genes flanking the IGR.(B) PCR amplified cDNA from IGRs run on a 1.0% agarose gel (Lane +). cDNA samples without reverse transcriptase (Lane −) and gDNA (Lane g) were used as negative and positive controls respectively.