Table 1.
Hippocampal stereological estimations.
| Hippocampal fields | Non-separated | PMS | |||||||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| N° cells (104) | Volume (108) | Area (105) | N° cells/Area | N° cells (104) | Volume (108) | Area (105) | N° cells/Area | ||
|
|
|
||||||||
| DG | Gr | 7.7 ± 1.6 | 1.3 ± 0.2 | 8.0 ± 1.5 | 0.1 ± 0.02 | 8.0 ± 1.0 | 1.2 ± 0.1 | 5.7 ± 0.4 | 0.1 ± 0.01 |
| Mol | - | 2.1 ± 0.5 | 12.7 ± 3.1 | - | - | 1.8 ± 0.1 | 9.9 ± 0.9 | - | |
| Pol | - | 1.3 ± 0.3 | 7.2 ± 1.8 | - | - | 0.7 ± 0.1 | 3.6 ± 0.7 | - | |
| Total | - | 4.7 ± 0.6 | 27.9 ± 5.9 | - | - | 3.7 ± 0.4 | 19.2 ± 2.0 | - | |
| CA1 | Pyr | 4.4 ± 0.5 | 1.0 ± 0.08 | 7.3 ± 0.5 | 0.06 ± 0.008 | 4.5 ± 0.4 | 1.1 ± 0.06 | 5.7 ± 0.3* | 0.07 ± 0.009 |
| Or | - | 1.9 ± 0.1 | 10.2 ± 0.9 | - | - | 1.8 ± 0.1 | 10.2 ± 0.8 | - | |
| Rad | - | 1.8 ± 0.1 | 10.1 ± 1.0 | - | - | 1.7 ± 0.1 | 9.9 ± 0.7 | - | |
| Total | - | 4.7 ± 0.2 | 27.6 ± 2.3 | - | - | 4.6 ± 0.2 | 25.8 ± 1.7 | - | |
| CA3 | Pyr | 4.4 ± 0.4 | 1.6 ± 0.1 | 10.4 ± 1.3 | 0.04 ± 0.005 | 4.6 ± 0.5 | 1.2 ± 0.1* | 7.2 ± 0.7 | 0.06 ± 0.007 |
| Or | - | 2.3 ± 0.3 | 12.6 ± 1.9 | - | - | 1.5 ± 0.1 | 8.5 ± 0.7 | - | |
| Rad | - | 1.5 ± 0.1 | 8.8 ± 1.0 | - | - | 1.2 ± 0.1 | 6.5 ± 0.8 | - | |
| Total | - | 5.4 ± 0.4 | 31.8 ± 3.8 | - | - | 3.9 ± 0.3* | 22.2 ± 1.9* | - | |
PMS= prolonged maternal separated mice
DG= Dentate gyrus; CA1= Cornus Ammonis 1; CA3=Cornus Ammonis 3
Gr=granular cells and Pyr=pyramidal cell. Mol=molecular layer; Pol=polymorphic layer; Or=oriens layer; Rad=stratum radiatum layer. Total=sum of each of the hyppocampal field layers.
*
p<0.05 versus the non-separated group by Unpaired Student t –test. N=7 for non-separated and PMS groups. Data are expressed as mean ± standard error of the mean (SEM).