Skip to main content
. Author manuscript; available in PMC: 2016 Jul 30.
Published in final edited form as: J Immunol. 1999 Aug 15;163(4):2187–2192.

FIGURE 1.

FIGURE 1

CTS suppresses rhIL-1β-dependent NO production in chondrocytes. A, Chondrocytes (5 × 105 cells/well) grown in Flexercell plates were subjected to: medium alone (○), CTS alone (●), rhIL-1β (1 ng/ml) (□), or CTS in the presence of IL-1β (■), for 2, 4, 12, 24, 48, 72, or 96 h. At the end of each incubation period, the culture supernatants were assayed for the presence of nitrite. B and C, Suppression of NO production by CTS at various concentrations of rhIL-1β. Cells were subjected to rhIL-1β (0, 0.1, 0.5, 1, 5, or 10 ng/ml) in the absence (open bars) or presence of CTS (filled bars). NO accumulation in the supernatants was measured after 24 or 96 h of activation. Each point represent means ± SEM of triplicate samples. *, p < 0.05 relative to cells treated with IL-1β alone.