Fig 5.

qPCR expression profiles of (A) myogenin and myosin heavy chain at days 0–9 of C2C12 differentiation; (B) glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine-guanine phosphoribosyltransferase and ribosomal protein S12 reference genes at days 0–9 of C2C12 differentiation. Analysis of gene expression was done after normalization to single reference gene (TATA box-binding protein) and the fold changes in gene expression were expressed relative to gene expression in undifferentiated cells. Results are expressed as mean ± standard deviation, (n = 3). Data were analyzed by Kruskal-Wallis nonparametric test followed by Dunn's test for multiple comparisons. a = significant difference in myogenin expression between day 0 and day 3 (P < 0.01); b = significant difference in myosin heavy chain expression between day 0 and day 7 (P < 0.01); c = significant difference in ribosomal protein S12 expression between day 0 and day 5 (P < 0.05). mRNA, messenger RNA.