Skip to main content
. 2016 Aug;174:140–160.e14. doi: 10.1016/j.trsl.2016.03.015

Fig 6.

Fig 6

Uptake of simvastatin lactone (SVL) and simvastatin hydroxy acid (SVA) by undifferentiated and differentiated C2C12 cells. The uptake was determined after incubation of the cells with 1 μmol/L of SVL or SVA in Dulbecco's modified eagle medium culture medium of different pH levels (6.8–7.8) at 37°C for 6 hours. Results are expressed as nanomoles per milligram of protein ±standard deviation, (n = 5). Data were analyzed by 2-way analysis of variance followed by Bonferroni's post hoc test. (A) Total simvastatin (lactone + hydroxy acid) recovered by undifferentiated C2C12 cells after treatment with 1 μmol/L SVL. a = pH 6.8 vs pH 7.4 (P < 0.01); pH 6.8 vs pH 7.8 (P < 0.001); b = pH 6.8 vs pH 7.4, 7.8 (P < 0.001); c = pH 6.8 vs pH 7.8 (P < 0.05). (B) Total simvastatin recovered by differentiated C2C12 cells after treatment with 1 μmol/L SVL. a = pH 6.8 vs pH 7.4, 7.8 (P < 0.001); pH 7.4 vs pH 7.8 (P < 0.01); b = pH 6.8 vs pH 7.4 (P < 0.01); pH 6.8 vs pH 7.8 (P < 0.001); c = pH 6.8 vs pH 7.4, 7.8 (P < 0.01). (C) SVA recovered by undifferentiated C2C12 cells after treatment with 1 μmol/L SVA (no SVL was recovered in this experiment). a = pH 6.8 vs pH 7.4 (P < 0.01); pH 6.8 vs pH 7.8 (P < 0.001); b = pH 6.8 vs pH 7.4, 7.8 (P < 0.001). (D) Total simvastatin recovered by differentiated C2C12 cells after treatment with 1 μmol/L SVA. a = pH 6.8 vs pH 7.4, 7.8 (P < 0.001); b = pH 6.8 vs pH 7.4, 7.8 (P < 0.001); pH 7.4 vs pH 7.8 (P < 0.05); c = pH 6.8 vs pH 7.4, 7.8 (P < 0.001); pH 7.4 vs pH 7.8 (P < 0.01); d = pH 6.8 vs pH 7.4, 7.8; pH 7.4 vs pH 7.8 (P < 0.001).