Supplementary Fig 11.

Messenger RNA expression of (A) ribosomal protein S12, (B) hypoxanthine phosphoribosyltransferase, (C) TATA box-binding protein, and (D) glyceraldehyde-3-phosphate dehydrogenase reference genes. Results are expressed as fold changes in gene expression relative to the baseline level observed with undifferentiated C2C12 maintained at physiological pH. Each reference gene was normalized to the other 3 reference genes. Data are presented as mean ± standard deviation, (n = 4) and were analyzed 2-way analysis of variance followed by Bonferroni's post hoc test, ^{∗∗∗, §§§, †††}P < 0.001, ^{∗∗, §§}P < 0.01, ^{∗, §, †}P < 0.05 compared with messenger RNA expression level in undifferentiated cell samples of pH 7.4.