Table I.
The pH dependence of the hydrolysis of the lactone form of statins
| Sample pH | Simvastatin lactone (half-life, hours) |
Pravastatin lactone (half-life, hours) |
||||
|---|---|---|---|---|---|---|
| PBS | Human plasma | DMEM | PBS | Human plasma | DMEM | |
| 6.8 | 26.68 ± 0.97 | 36.51 ± 4.37∗,† | 21.42 ± 2.35∗ | 21.22 ± 1.75 | 8.84 ± 0.31∗,† | 17.30 ± 4‡ |
| 7.0 | 16.92 ± 0.86 | 21.30 ± 2.50∗ | - | 12.96 ± 1.38 | 7.39 ± 0.22 | - |
| 7.2 | 13.68 ± 0.71 | 14.46 ± 3.55 | 11.70 ± 0.50 | 9.36 ± 1.57 | 6.61 ± 0.95§,† | 11.89 ± 2.71 |
| 7.4 | 12.86 ± 0.31 | 10.45 ± 0.38 | 7.40 ± 0.37∗ | 6.89 ± 0.41 | 6.85 ± 0.54‖ | 9.72 ± 1.42§ |
| 7.6 | 9.58 ± 1.67 | 9.16 ± 1.77 | - | 5.59 ± 0.49 | NA | - |
| 7.8 | 10.23 ± 1.18 | 8.61 ± 3.38 | 7.09 ± 0.30 | 4.98 ± 0.44 | NA | 10.66 ± 0.81‡ |
Abbreviations: DMEM, Dulbecco's modified eagle medium; NA, nonapplicable; PBS, phosphate buffer saline.
Data are presented as mean ± SD of 6 replicates. Statistical analysis was done by 2-way analysis of variance followed by Bonferroni's post hoc test.
∗
Significant difference from PBS samples (P < 0.001).
†
Significant difference from DMEM samples (P < 0.001).
‡
Significant difference from PBS samples (P < 0.01).
§
Significant difference from DMEM samples (P < 0.05).
‖
Significant difference from DMEM (P < 0.05).