Table II.

Cytotoxicity of statins to C2C12 cells after 72 hours of treatment in medium of different pH. Data are presented as mean ± SD of 3 experiments, 8 replicates per experiment

Statin	C2C12 cells	IC50 values of statins (μmol/L)
Acidic medium	Neutral medium	Alkaline medium
Simvastatin lactone	Undifferentiated cells	0.44 ± 0.02∗	0.58 ± 0.02†	1.13 ± 0.04‡
Differentiated cells	15.79 ± 0.77∗,§	40.76 ± 1.75†,§	56.99 ± 1.86‡,§
Simvastatin hydroxy acid	Undifferentiated cells	2.22 ± 0.04∗	4.35 ± 0.09†	16.85 ± 0.48‡
Differentiated cells	65.41 ± 2.69§	NA	NA
Pravastatin lactone	Undifferentiated cells	85.59 ± 2.05	NA	NA
Differentiated cells	109.9 ± 3.74§	NA	NA

Abbreviations: NA, non-applicable; SD, standard deviation.

Data are presented as mean ± SD of 3 experiments, 8 replicates per experiment. Statistical analysis was done by 2-way analysis of variance followed by Bonferroni's post hoc test.

^∗Significant difference from neutral and alkaline medium (P < 0.001).

^†Significant difference from acidic and alkaline medium (P < 0.001).

^‡Significant difference from acidic and neutral medium (P < 0.001).

^§Significant difference from undifferentiated cells (P < 0.001).