SupplemenItary Table III.
Primers used for gene expression quantification of cellular transporters
| Gene | Direction | Primer sequence (5′-3′) | Melting temperature (°C) | Amplicon length (bp)∗ |
|---|---|---|---|---|
| MRP1 | Forward | GTTATGGAAGCCTGTGCCCT | 60.03 | 296 |
| Reverse | ATGACATCCACTTGGGGCAG | 60.03 | ||
| MRP4 | Forward | CCTGGTGAGTTGTTAGCCGT | 59.97 | 326 |
| Reverse | AGGTTCACCCGAGCTTTCTG | 59.96 | ||
| MRP5 | Forward | CTTCCTGGGATCAGCGGTTT | 60.04 | 244 |
| Reverse | GCTCTGAAAGTACCCGGCTT | 60.04 | ||
| OATP1a4 | Forward | AACTCCCATCATGCCCTTGG | 60.03 | 246 |
| Reverse | CCTGCACAGACCAAAAAGCC | 59.97 | ||
| OATP2b1 | Forward | GCCAGAAGGAGGCATCAACT | 60.03 | 233 |
| Reverse | TTAAAGGCTCGTGCTGGGAG | 60.04 | ||
| OATP1a4 | Forward | CTCACTTGGCTGTGATTCTTGT | 58.86 | 254 |
| Reverse | TCTTCTGATTGTCTCCAAATGTCT | 57.88 | ||
| OATP2b1 | Forward | TCATCCTGAGAGGTGTGAAGAAAG | 60.02 | 203 |
| Reverse | TATGAATCGGTTTCGGAGCAGG | 60.48 | ||
| OATP1a4 | Forward | CCACGTCTGTAGTTGGGCTT | 59.97 | 291 |
| Reverse | TCACACACTCTGTTGGGTCT | 58.50 | ||
| OATP2b1 | Forward | TGCCAGAAGGAGGCATCAAC | 60.32 | 346 |
| Reverse | ACCAGCAAGAAGATGGGGTG | 59.96 | ||
| Gapdh | Forward | TGTGAACGGATTTGGCCGTA | 59.96 | 159 |
| Reverse | ACTGTGCCGTTGAATTTGCC | 59.97 | ||
| Hprt | Forward | AGTCCCAGCGTCGTGATTAG | 59.54 | 170 |
| Reverse | TGATGGCCTCCCATCTCCTT | 60.33 | ||
| Rps12 | Forward | ACAAGAGGTGCTGAAGACCG | 59.97 | 275 |
| Reverse | CTACGCAACTGCAACCAACC | 60.04 | ||
| Tbp | Forward | CTCAGTTACAGGTGGCAGCA | 59.96 | 118 |
| Reverse | GCACAGAGCAAGCAACTCAC | 60.04 |
∗
bp = base pairs.