Supplementary Table V.
Intraday and interday precision and accuracy of HPLC methods used for determination statins in PBS samples. Both intraday and interday assay variability values were within acceptance criteria for all tested compounds at the 4 QC levels
| Statins | Quality control levels |
Intraday (n = 6) |
Interday (n = 6) |
|||
|---|---|---|---|---|---|---|
| Concentration (μmol/L) | Concentration (μg/mL) | Precision (RSD%)∗ | Accuracy (RE%)† | Precision (RSD%) | Accuracy (RE%) | |
| Simvastatin lactone | LLOQ (0.156) | 0.065 | 1.36 | −7.04 | 7.91 | 15.80 |
| LQC (5) | 2.09 | 4.51 | −0.81 | 3.92 | −11.04 | |
| MQC (60) | 25.12 | 11.89 | −8.30 | 13.49 | 9.62 | |
| HQC (120) | 50.23 | 10.39 | −6.69 | 10.16 | −8.67 | |
| Simvastatin hydroxy acid | LLOQ (0.313) | 0.142 | 3.75 | −6.69 | 11.60 | −19.38 |
| LQC (5) | 2.26 | 8.39 | −13.58 | 13.55 | 6.56 | |
| MQC (60) | 27.21 | 12.01 | 9.41 | 9.88 | 6.14 | |
| HQC (120) | 54.43 | 9.65 | −1.8 | 10.75 | −0.53 | |
| LLOQ (0.234) | 0.095 | 4.16 | 7.06 | 19.92 | −2.66 | |
| Pravastatin lactone | LQC (10) | 4.06 | 2.45 | 9.66 | 2.71 | 14.11 |
| MQC (100) | 40.65 | 5.92 | 9.35 | 7.05 | −3.10 | |
| HQC (200) | 81.30 | 5.28 | 14.57 | 6.18 | −1.46 | |
| Pravastatin hydroxy acid | LLOQ (0.469) | 0.209 | 2.12 | 19.99 | 14.32 | −15.86 |
| LQC (10) | 4.46 | 3.52 | 8.81 | 8.39 | 13.08 | |
| MQC (100) | 44.65 | 4.50 | 7.56 | 4.86 | 1.79 | |
| HQC (200) | 89.30 | 12.69 | 4.79 | 4.93 | 2.29 | |
Abbreviations:HQC, high concentration quality control; LLOQ, lower limit of quantification; LQC, low concentration quality control; MQC, medium concentration quality control; PBS, phosphate buffer saline.
∗
Relative standard deviation (RSD%) = (standard deviation [SD]/mean of obtained concentration) × 100.
†
Relative error (RE%) = ([measured concentration−nominal concentration]/nominal concentration) × 100.