Supplementary Table VII.
Intraday and interday precision and accuracy of LC MS/MS methods used for determination of statins in cell lysate samples. Both intraday and interday assay variability values were within acceptance criteria for all tested compounds at the 4 QC levels
| Statins | Quality control levels |
Intraday (n = 6) |
Interday (n = 6) |
|||
|---|---|---|---|---|---|---|
| Concentration (nmol/L) | Concentration ng/mL | Precision (RSD%)∗ | Accuracy (RE%)† | Precision (RSD%) | Accuracy (RE%) | |
| Simvastatin lactone | LLOQ (5) | 2.09 | 14.34 | 10.06 | 15.89 | 9.60 |
| LQC (20) | 8.37 | 12.35 | 7.44 | 13.70 | −1.79 | |
| MQC (200) | 83.72 | 12.91 | 5.47 | 10.71 | 9.24 | |
| HQC (2000) | 837.17 | 12.45 | 8.30 | 10.52 | 3.93 | |
| Simvastatin hydroxy acid | LLOQ (2.5) | 1.13 | 7.215 | −3.17 | 9.30 | 0.16 |
| LQC (20) | 9.07 | 4.69 | 8.75 | 5.18 | 9.52 | |
| MQC (200) | 90.72 | 5.12 | 3.51 | 6.59 | 4.77 | |
| HQC (2000) | 907.24 | 8.38 | −5.44 | 8.19 | −7.57 | |
| Pravastatin lactone | LLOQ (5) | 2.03 | 9.98 | 14.16 | 6.96 | 10.00 |
| LQC (20) | 8.13 | 11.46 | −4.73 | 11.64 | −2.20 | |
| MQC (200) | 81.3 | 11.36 | 0.03 | 9.43 | 3.72 | |
| HQC (2000) | 813 | 7.3 | −0.20 | 6.28 | −0.11 | |
| Pravastatin hydroxy acid | LLOQ (5) | 2.23 | 17.04 | 7.30 | 12.93 | 11.62 |
| LQC (20) | 8.93 | 11.76 | −6.32 | 12.38 | −6.87 | |
| MQC (200) | 89.30 | 12.28 | −9.45 | 12.01 | −3.61 | |
| HQC (2000) | 893.02 | 11.10 | 0.08 | 8.79 | 0.03 | |
Abbreviations:HQC, high concentration quality control; LLOQ, lower limit of quantification; LQC, low concentration quality control; MQC, medium concentration quality control.
∗
Relative standard deviation (RSD%) = (standard deviation [SD]/mean of obtained concentration) × 100.
†
Relative error (RE%) = ([measured concentration−nominal concentration]/nominal concentration) × 100.