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. 2016 Jul 28;166(3):664–678. doi: 10.1016/j.cell.2016.06.015

Figure 7.

Figure 7

NPC Insertion in the Context of Embryonic Development

(A) AL are abundant throughout the ∼120 min of syncytial development but diminish from the cortical layer in interphase 14, concomitant with cellularization and the transcriptional activation at zygotic induction.

(A′) NE-NPCs are laterally mobile and all along the NE in the syncytial blastoderm, but immobilize and cluster at the NE starting with cellularization.

(B) In each precedent interphase of the syncytial blastoderm, AL number oscillates within the cortical nuclear layer on a timescale of ∼10 min, with increasingly longer interphases in each cycle. Inverse to AL-NPC number, which decreases in each interphase, NE-NPC number increases together with nuclear surface expansion.

(C and D) AL-NPCs insertion to the NE occurs in the range of 1–2 min. It involves an open NE and batch insertion of AL-NPCs within a proximal ER sheet (D). Lateral mobility allows NE-redistribution of inserted NPCs. Insertion of subsequent ER sheets augments nuclear surface (C).