Figure 4.

Expression of Cu/Zn-SOD, Mn-SOD, catalase, Bcl-2, and Bax proteins in the presence or absence of melatonin and/or 2-DG in rat insulinoma INS-1E cells. INS-1E cells were incubated in RPMI 1640 medium supplemented with 2% FBS with/without melatonin (100 μM) and/or 2-DG (5 mM) for 24 hr at 37°C with 5% CO₂. Cu/Zn-SOD, Mn-SOD, and catalase proteins were analyzed by Western blot (a, e). The relative amounts of Cu/Zn-SOD (b), Mn-SOD (c), catalase proteins (d), Bcl-2 (f), and Bax proteins (g) were quantified in Section 2. Data represent mean ± SD of three experiments (n = 3). ^∗ p < 0.05, ^∗∗∗ p < 0.001 versus 2% FBS; ^# p < 0.05, ^## p < 0.01, and ^### p < 0.001, melatonin versus melatonin and/or 2-DG.