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. 2016 Jul 7;16(4):1039–1054. doi: 10.1016/j.celrep.2016.06.046

Figure 1.

Figure 1

Mll1 Displays a Widespread Expression Pattern during Development

(A and B) The qPCR for Mll1 expression was performed on (A) whole tissues (n = 2–6) or (B) sorted HC populations (n = 1–5). HSPC, CD34+ ckit+; HC, CD45+ CD34− CD19−; HSC/MPP, Lin− B220− CD19− ckit+ CD45+ Sca1+ Flt3− IL7R−; LMPP, Lin− B220− CD19− ckit+ CD45+ Sca1+ Flt3+; ESLAM, EPCR+ CD45+ CD150+ CD48−. Error bars indicate SEM.

(C–G) Immunohistochemistry was performed showing Mll1 protein presence in the (C) E11 AGM (Mll1, red; CD34, green; DAPI, blue; inset, enlargement of boxed-in area; green arrowhead, Mll1 expression in mesonephric ducts; white arrowhead, expression in circulating cells; yellow arrowhead, expression in sub-aortic mesenchymal cells; DA, dorsal aorta; M, myotome; SNS, sympathetic nervous system; UGR, urogenital ridges; ×20 objective); (D) E11 dorsal aorta (Mll1, red; ckit, white; DAPI, blue; yellow arrowhead, Mll1 expression in the sub-aortic mesenchyme; white arrowhead, expression in circulating ckit+ cells; white arrow, expression in ckit+ intra-aortic cluster cells; ×100 objective); (E) E11 dorsal aorta; Mll1, red; ckit, white; CD34, green; DAPI, blue; white arrow, expression in ckit+CD34+ intra-aortic cluster cells; ×100 objective); (F) E14 FL (Mll1, red; ckit, white; CD34, green; DAPI, blue; white arrow, cell showing co-expression of all three markers; ×63 objective); and (G) E12 placenta (Mll1, red; CD34, green; DAPI, blue; UA, umbilical artery; ×5 objective).