Skip to main content
. 2016 Jul 7;16(4):1039–1054. doi: 10.1016/j.celrep.2016.06.046

Figure 3.

Figure 3

Mll-AF4 Expression Leads to a Mild Myeloid Expansion

(A) Myeloid colony formation from E12 FL cells of the indicated genotypes is shown (n = 4–5).

(B) CD11b and Gr1 expression on cells isolated from E12 FL myeloid colony plates of the indicated genotypes is shown.

(C) Myeloid colony formation from E14 FL cells of the indicated genotypes is shown (n = 3–7).

(D) CD11b and Gr1 expression on cells isolated from E14 FL myeloid colony plates of the indicated genotypes is shown.

(E) Myeloid colony formation from sorted E12 FL HSC/MPP (Lin− B220− CD19− ckit+ CD45+ Sca1+ Flt3− IL7R−; n = 2, VEC; n = 4, MA4xVEC) and LMPP (Lin− B220− CD19− ckit+ CD45+ Sca1+ Flt3+) cells is shown (n = 3 for both genotypes).

(F) Myeloid colony formation from sorted E14 FL HSC/MPP (n = 5, VEC; n = 7, MA4xVEC) and LMPP cells is shown (n = 5, VEC; and n = 4, MA4xVEC).

(G) Replating potential of E14 FL HSC/MPP of indicated genotypes in myeloid conditions is shown (n = 3–5).

(H) Myeloid colony formation from total adult BM cells of indicated genotypes is shown (n = 1, WT; n = 3, VEC; n = 2, MA4; and n = 4, MA4xVEC).

(I) Replating potential of adult BM HSC/MPP of indicated genotypes in myeloid conditions is shown (n = 3, VEC; n = 1, MA4; and n = 2, MA4xVEC).

Statistical analysis was performed using a one-way ANOVA with Tukey’s multiple comparisons test (∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.05). Error bars indicate SEM.