Table 2.
Sequence of the primers and the amplicon sizes for the detection of 11 virulence-associated Brucellamelitensis
| Genes | Primer Sequences (5’ 3’) | Amplicon Sizes | References |
|---|---|---|---|
| cbg | F: GAATTCGCCAATGAGGAAAA R: ACGATATCGGATGCGAAAAG |
575 bp | 16 |
| mviN | F: GCAGATCAACCTGCTCATCA R: GCCATAGATCGCCAGAATA |
344 bp | 16 |
| manA | F: TCGATCCAGAAACCCAGTTC R: CATACACCACGATCCACTGC |
271 bp | 16 |
| manB | F: GGCTGGTTCGAGAATATCCA R: CAATCGCATACCCTGGTCTT |
228 bp | 16 |
| wbkA | F: GAGCGCTTAGGAATGCTGAT R: CTCCTAGGTTCCAGCCCTTT |
309 bp | 16 |
| omp25 | F: CGTACCTCACGGCTGGTATT R: CGTACCGGCCAGATCATAGT |
188 bp | 16 |
| omp31 | F: GCTGCTCCTGTTGACACCTT R: GCTGAAATCGAACCCGTAAC |
257 bp | 16 |
| znuA | F: CTGGGTCCGAGCATGTTTAT R: AGGCATCGAGTTTTTCTCCA |
465 bp | 16 |
| bvfA | F: CCCTTCGTCGATGTGCTGA R: CCGCGCTGATTTCATCGCTG |
1282 bp | 17 |
| virB | F: CGCTGATCTATAATTAAGGCTA R: TGCGACTGCCTCCTATCGTC |
881 bp | 17 |
| ure | F: GCTTGCCCTTGAATTCCTTTGTGG R: ATCTGCGAATTTGCCGGACTCTAT |
2100 bp | 17 |