Table 4.
Biological processes upregulated and downregulated as derived from DAVID algorithm based on proteins significantly differing in abundance by spectral counts and/or peak area in Sp2-cKO mice
| Biological processes altered in Sp2-cKO
| |||||
|---|---|---|---|---|---|
| Pathway | Genes | p value | Fold enrichment | Fisher exact | Direction in KO |
| Mitotic cell cycle | 7 | 8.10E−04 | +6.2 | 1.20E−04 | Upregulated |
| Organelle fission | 6 | 2.00E−03 | +6.6 | 2.80E−04 | Upregulated |
| Cell cycle phase | 7 | 3.60E−03 | +4.6 | 7.50E−04 | Upregulated |
| Cell cycle process | 7 | 8.60E−03 | +3.9 | 2.10E−03 | Upregulated |
| Microtubule-based movement | 4 | 1.10E−02 | +8.6 | 1.20E−03 | Upregulated |
| Alcohol catabolic process | 3 | 3.50E−02 | +10 | 3.30E−03 | Upregulated |
| Carbohydrate catabolic process | 3 | 5.20E−02 | +8 | 6.20E−03 | Upregulated |
| Cellular carbohydrate metabolic process | 5 | 6.10E−02 | +3.3 | 1.70E−02 | Upregulated |
| Cellular macromolecule metabolic process | 27 | 9.50E−02 | +1.3 | 7.00E−02 | Upregulated |
| Electron transport chain | 7 | 3.40E−05 | −11.2 | 2.90E−06 | Downregulated |
| Generation of precursor metabolites and energy | 9 | 9.00E−03 | −6.2 | 1.40E−05 | Downregulated |
| Gas transport | 3 | 2.20E−03 | −41.4 | 4.60E−05 | Downregulated |
| Cellular macromolecule metabolic process | 38 | 2.90E−03 | −1.5 | 1.90E−03 | Downregulated |
| Ribosome biogenesis | 5 | 3.30E−03 | −8 | 4.00E−04 | Downregulated |
| Transport | 23 | 6.20E−03 | −1.8 | 3.40E−03 | Downregulated |
| Cellular catabolic process | 12 | 6.40E−03 | −2.5 | 2.40E−03 | Downregulated |
| Response to dsRNA | 3 | 6.40E−03 | −24.5 | 2.40E−04 | Downregulated |
| Protein localization | 11 | 7.90E−03 | −2.6 | 2.90E−03 | Downregulated |
| Protein transport | 10 | 9.20E−03 | −2.8 | 3.20E−03 | Downregulated |
| Establishment of protein localization | 10 | 9.60E−03 | −2.7 | 3.40E−03 | Downregulated |
| Cellular nitrogen compound metabolic process | 27 | 1.20E−02 | −1.6 | 7.70E−03 | Downregulated |
| Nucleobase, nucleoside, nucleotide, and nucleic acid metabolic process | 25 | 1.80E−02 | −1.6 | 1.10E−02 | Downregulated |
| Once-carbon metabolic process | 4 | 2.70E−02 | −6.1 | 4.10E−03 | Downregulated |
| Cell morphogenesis | 6 | 2.80E−02 | −3.5 | 7.50E−03 | Downregulated |
| Embryonic development ending in birth or egg hatching | 7 | 2.90E−02 | −3 | 9.40E−03 | Downregulated |
| Embryonic development | 9 | 3.00E−02 | −2.4 | 1.20E−02 | Downregulated |
| Gene expression | 22 | 3.40E−02 | −1.5 | 2.10E−02 | Downregulated |
| Blastocyst development | 3 | 3.60E−02 | −10 | 3.40E−03 | Downregulated |
| Cellular component morphogenesis | 6 | 4.40E−02 | −3.1 | 1.30E−02 | Downregulated |
| Macromolecule catabolic process | 8 | 6.80E−02 | −2.2 | 2.90E−02 | Downregulated |
| Chromosome organization | 6 | 7.10E−02 | −2.7 | 2.50E−02 | Downregulated |
| Cellular response to stress | 6 | 7.10E−02 | −2.7 | 2.50E−02 | Downregulated |
| Cellular biosynthetic process | 23 | 7.90E−02 | −1.4 | 5.40E−02 | Downregulated |
| Cellular macromolecule localization | 5 | 8.40E−02 | −3 | 2.60E−02 | Downregulated |
| Regulation of binding | 3 | 8.80E−02 | −6 | 1.40E−02 | Downregulated |
| Intracellular transport | 6 | 8.80E−02 | −2.5 | 3.30E−02 | Downregulated |