Figure 3.

Effects of GvHD on ferritin and iron metabolism in mice. In C57BL/10 mice transplanted with Balb/c splenocytes and GvHD, a significantly higher amount of liver iron was found on day+9 (A); *P<0.05; unpaired t-test, two tailored). In accordance, mRNA expression of several genes related to iron metabolism and macrophage activation was changed during hepatic GvHD at day +14 (Balb/c →C57B6/N) (B); data are shown as mean±s.e.m; *P<0.05 and ***P<0.001; t-test; 4 mice per group. The expression of some proteins related to iron balance (Cp, Lcn2 and Hmox1) and others related to macrophage activation (Crp and Lcp1) was also significantly up regulated in allogeneic animals (LP/J→C57B6/N) (C); data are shown as mean±s.e.m; *P<0.05 and **P<0.01 and ***P<0.001; t-test; 4 mice per group. The proteomics analysis indicated that 2 days after BMT, compared to the syngeneic mice, macrophage migration inhibitory factor (Mif) was highly upregulated in the allogeneic livers (LP/J→C57B6/N) (D).