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. 2016 Jul 28;166(3):582–595. doi: 10.1016/j.cell.2016.06.024

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© 2016 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Affinity of Patient-Derived mAbs

(A) Amino acid sequences of 26B9 and 19D11 anti-IFN antibodies aligned with closest corresponding germline IgV_H, D_H, J_H, V_L, and J_L sequences. Identities highlighted in blue; conservative mutations in yellow; non-conservative in white; CDRs underlined in red.

(B) Plasmon resonance data: antibodies 19D11 and 26B9 were immobilized on Biacore chips; different concentrations of recombinant human IFNα2b, IFNα4, IFNα14, and IFNω were passed over; response units were recorded; and dissociation constants (K_D) calculated.

(C) Scatter chart of K_D values derived from (B).

(D) Binding determined by LIPS of APS1/APECED-derived mAbs and of germline counterparts to IFNα2, IFNα8, IFNα14 (19D11, 50E11, and 26B9), IL22 (35G11 and 30G1), and IL20 (20A10 and 2A11). Binding to immobilized IL17F (17E3 and 9A2) was determined by ELISA.