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In Vitro Neutralization
(A) IC50 analysis of APS1/APECED-derived anti-IFN mAbs 19D11 and 26B9 in HEK293T MSR cells transfected with ISRE dual-luciferase reporter constructs and treated with IFNα subtypes shown. Error bars correspond to SEM of multiple measurements.
(B–D) IFN-induced STAT1 tyrosine phosphorylation detected by western blot and normalized to total STAT1 or to tubulin levels as loading controls. Vertical lines in (B) and (C) denote cropped lanes.
