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. 2016 Aug 1;3(4):ENEURO.0150-16.2016. doi: 10.1523/ENEURO.0150-16.2016

Figure 1.

Figure 1.

OSN apoptosis in Camk2a-hAPP mice. A, Strategies to generate mutant lines with olfactory-specific or broad CNS-specific overexpression of hAPP by using OMP or Camk2a promoter, respectively, and the tTA-TetO system. B, Diagram showing the organization of the OE, with markers for mature and immature OSNs highlighted. C, Left panels, Camk2a (red) and hAPP (green) immunohistochemical signal in the olfactory epithelium from 3-week-old Camk2a-hAPP and tetO-hAPP mice, respectively. Camk2a was broadly expressed in OSNs and mostly colocalized with hAPP in Camk2a-hAPP mice. Right panels, GAP43 (immature OSN marker, red) and OMP (mature OSN marker, green) immunohistochemical signal in the epithelium. Note that Camk2a-hAPP animals had less mature OSNs and thinner epithelia than controls. D, E, The 3-week-old Camk2a-hAPP animal had many more cleaved caspase-3-positive cells in the epithelium than the control animal (D), which colocalized in hAPP-expressing neurons (E, arrowheads). Scale bars: C, E, 20 µm; D, 100 µm.