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. 2016 Jul 27;6(7):160018. doi: 10.1098/rsob.160018

Figure 3.

Figure 3.

Coexpression of a constitutively active Gs mutant in M71 OSNs does not induce a posterior shift of glomeruli. (a) Schematic overview of the targeted M71 mutations. (b,c) Wholemount intrinsic GFP fluorescence of the dorsal bulb (3wo), showing projection sites of lateral M71-caGs-GFP axons (green). (b) Glomerular formation is normal (16/36 bulbs). (c) Axons project to the correct A-P position, but do not converge into glomeruli (20/36 bulbs). (d) Wholemount intrinsic GFP fluorescence in M71-M71-GFP mice (3wo). M71-M71-GFP axons always formed glomeruli (14/14 bulbs). (e) Wholemount intrinsic GFP and RFP fluorescence in M71-RFP × M71-caGs-GFP mice (3wo, n > 10). M71-RFP (red) and M71-caGs-GFP (green) axons converge, but segregate and form compartmentalized glomeruli. (f) Wholemount GFP and RFP fluorescence in M71-RFP × M71-M71-GFP mice (3wo, n > 10). M71-RFP (red) and M71-M71-GFP (green) axons converge, but segregate and form compartmentalized glomeruli. Scale bars, 250 µm (b,c,d), 50 µm (e,f).