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. 2016 Jul 27;6(7):160018. doi: 10.1098/rsob.160018

Figure 8.

Figure 8.

Ectopically expressed O/E2-M71 elicits both agonist-dependent and -independent neuronal activities. (a–c) Representative traces of perforated patch-clamp recordings in the voltage-clamp mode in response to the indicated ligands in (a) random OSNs or (b) MOR23(RDY) → M71-caGs-Tomato OSNs in O/E2-M71-GFP heterozygous mice; (c) random OSNs in a WT mouse. (d,e) Dose–response curves of acetophenone stimulated (d) random OSNs or (e) MOR23(RDY) → M71-caGs-Tomato OSNs in O/E2-M71-GFP heterozygous mice. The curve for the average is in black. (f) A threshold frequency histogram for random OSNs (white bar, n = 10) or MOR23(RDY) → M71-caGs-Tomato OSNs (black bar, n = 10) in O/E2-M71-GFP heterozygous mice, and M71-GFP OSNs in O/E2 WT mice (grey bar, n = 6). This histogram represents the number of cells with a detection threshold in the 10−7, 10−6, 10−5 or 10−4 M range of stimulating concentration. (g) Representative traces of spontaneous activities recorded through perforated patch-clamp in the current clamp mode from random OSNs or MOR23(RDY) → M71-caGs-Tomato OSNs in O/E2-M71-GFP heterozygous animals. (h1) Mean spontaneous firing frequency and (h2) IF for the same OSN types described in (a–c). n = 6, 13, 10, respectively. Data are mean ± s.e.mb. One-way ANOVA and Tukey post test.