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. 2016 Jul 6;6(7):160029. doi: 10.1098/rsob.160029

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — (a) Total ion chromatogram (TIC) of acetylated sterols obtained by GC/MS from cultures grown for four days in ATCC 1034 medium. (b) The identities and chemical structures of the most abundant compounds are indicated. (c) C₁₈ HP-TLC autoradiography obtained from C. owczarzaki 4-day cultures supplemented with 0.5 µCi 4–¹⁴C-cholesterol. Lane I: 4–¹⁴C-cholesterol standard (0.005 µCi); lanes II and III: sterol extracts from two independent cultures. The scheme at the right represents the spots observed in lane III, for better interpretation. Numbering of the sterol species correspond to structures shown in (b). R_f, retention factor.