Table 1.
Sterol biosynthesis genes identified in C. owczarzaki.
| similarity to known sequences |
|||||||
|---|---|---|---|---|---|---|---|
|
Homo sapiens |
Saccharomyces cerevisiae |
Arabidopsis thaliana |
|||||
| gene description (vertebrate/yeast/plant) | protein ID | score (bits) | ID (%) | score (bits) | ID (%) | score (bits) | ID (%) |
| squalene monooxygenase, SMO (SQLE/ERG1/SQE) |
CAOG_07297 | 518 | 55 | 406 | 38 | 307 | 53 |
| oxidosqualene cyclase, OSC (LSS/ERG7/CAS1) |
CAOG_01478 | 801 | 54 | 511 | 39 | 629 | 47 |
| C14-demethylase (CYP51A1/ERG11/CYP51G1) |
CAOG_05595 | 561 | 52 | 294 | 37 | 322 | 38 |
| C14-reductase (TM7SF2/ERG24/FK) |
CAOG_07273 | 340 | 50 | 310 | 41 | 210 | 37 |
| C4-methyloxidase (SC4MOL/ERG25/SMO1,2) |
CAOG_07134 | 386 | 60 | 177 | 39 | 204 | 41 |
| 4α-carboxylase 3-dehydrogenase (NSDHL/ERG26/AT3βHSD) |
CAOG_05258 | 401 | 56 | 207 | 36 | 254 | 40 |
| hydroxysteroid dehydrogenase 7 (HSD17B7/ERG27/—) |
CAOG_04522 | 234 | 40 | 79 | 23 | — | — |
| C4-demethylase complex D (C14orf1/ERG28/ERG28) |
CAOG_04184 | 96.7 | 45 | 76.6 | 32 | 48.5 | 29 |
| 24-C-methyltransferase, SMT (—/ERG6/SMT1,2) |
CAOG_05252 | — | — | 316 | 49 | 367 | 52 |
| Δ8,7 isomerase (—/ERG2 /—) |
CAOG_04302 | — | — | 176 | 51 | — | — |
| CAOG_00613 | — | — | 85.5 | 33 | — | — | |
| Δ8,7 isomerase (EBP/—/HYD1) |
CAOG_04768 | 189 | 52 | — | — | 77.4 | 30 |
| C5-desaturase (SC5DL/ERG3/STE1) |
CAOG_00853 | 351 | 57 | 256 | 55 | 108 | 34 |
| C22-desaturase (—/ERG5/CYP710A1) |
CAOG_00150 | — | — | 364 | 39 | 263 | 35 |
| Δ24(28)-reductase (—/ERG4/—) |
CAOG_01185 | — | — | 318 | 40 | — | — |
| Δ24(25)-reductase (DHCR24/—/—) |
CAOG_04187 | 447 | 44 | — | — | 401 | 38 |
| Rieske C7-desaturasea (Nvd,DAF-36/—/—) |
CAOG_04775 | — | — | — | — | — | — |
| cyclopropyl isomerase (—/—/CPI1) |
CAOG_06065 | — | — | — | — | 187 | 38 |