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. 2016 Jul 20;6(7):160101. doi: 10.1098/rsob.160101

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — The effects on two subsequent preconditioning stimuli are additive. (a,b) Representative heart sections of transgenic cmlc2:DsRed2-nuc (red) fish labelled with the G1/S-phase marker MCM5 (green) at 7 dpt. Arrows indicate double-positive nuclei. (c) Experimental design. Fish were injected intraperitoneally (IP) with Zymosan 3 days before the thoracotomy procedure and cell-cycle activity was tested at 7 dpt. (d) Quantification of MCM5-positive CM nuclei at 7 dpt (n ≥ 4 hearts; ≥ 2 sections per heart; ***p < 0.001).