Table 2. Salivary Protein Presence in Breast Cancer Cell Lines and Exosomes.
| UniProt | Protein Name | Method of Identification | Presence in Cell Lines | Exosomes In Saliva | Exosomes in T.T. | Ref. |
|---|---|---|---|---|---|---|
| Genomic Integrity Related Proteins | ||||||
| P16403 | Histone H1.2 | MS | TT | 25 | ||
| Q8IUE6 | Histone H2A | MS | TT | 25 | ||
| Q99880 | Histone H2B type 1-L (H2B.c) | MS | TT | 25 | ||
| Q71DI3 | Histone H3.2 | MS | TT | 28 | ||
| P62805 | Histone H4 | MS | Yes | 73 | ||
| P12004 | Prolif. cell nuclear antigen | AA | T, TT | 8, 20, 77 | ||
| Molecular Chaperones/Heat Shock Proteins | ||||||
| Q6P5S2 | C6orf58 | 2D, MS | Yes | 85 | ||
| P27797 | Calreticulin | MS, AA | S, B, M, MD, TT | Yes | 8, 15, 16, 17, | |
| P11021 | Glucose-regulated protein | AA | S, B, M, MD, TT | Yes | Yes | 8, 15, 16, 17, 74, 85 |
| P11142 | Heat Shock 10 protein | MS | Yes | Yes | 74, 85 | |
| Q12988 | Heat Shock 27 protein | MS | B, M, MD, TT | 14, 15, 16 | ||
| P08107 | Heat Shock 70 protein | MS | B, M, T | Yes | Yes | 15, 16, 74, 85 |
| P05307 | Protein disulfide isomerase; p55 | AA | Yes | 8, 15, 16, 17, 85 | ||
| P07237 | Protein disulfide-isomerase | MS | B, M, MD, TT | 14, 15, 16 | ||
| P62937 | Peptidyl-prolyl cis-trans isom. A | MS | S, B, M, MD | Yes | Yes | 15, 16, 17, 74, 85 |
| Cell Growth Related Proteins | ||||||
| P63104 | 14-3-3 protein zeta/delta | AA | B, M, MD, TT | Yes | 8, 15, 18, 85 | |
| P00533 | epidermal growth factor receptor | E | S, M | Yes | Yes | 10, 17, 74, 85 |
| P29354 | Growth factor receptor protein 2 | AA | S, M, MD | Yes | 8, 17, 85 | |
| P04626 | epidermal growth factor receptor2 | WB, E | S | Yes | Yes | 10, 17, 74, 85 |
| P15531 | Metastatic process-associ. prot. | AA | M | 8 | ||
| Apoptosis Related Proteins | ||||||
| P31947 | 14-3-3 protein sigma | MS | TT | 18, 37 | ||
| P38936 | WAF-1 | AA, E | S | 8, 17, 39 | ||
| P04637 | protein 53 | E | TT | 8, 36 | ||
| P62988 | Ubiquitin | MS, WB | S, B | Yes | Yes | 15, 16, 17, 85 |
| Immunity Related Proteins | ||||||
| P61769 | Beta-2 microglobulin precursor | MS | S, B | Yes | Yes | 15, 16, 17, 74, 85 |
| Q8N4F0 | Long palate, lung and nasal epith. | MS | Yes | Yes | 74, 85 | |
| Q9UGM3 | GP 300 | MS | Yes | 85 | ||
| P01579 | Interferon gamma | AA | TT | 8, 57 | ||
| P01876 | Ig alpha1 chain C region | MS | Yes | 85 | ||
| P01877 | Ig alpha-2 chain C region | MS | Yes | 85 | ||
| P01857 | Ig gamma-1 chain C region | MS | Yes | 15, 85 | ||
| P01859 | Ig gamma-2 chain C region | MS | Yes | 85 | ||
| P01591 | Immunoglobulin J chain | MS | Yes | 85 | ||
| P05112 | Interleukin - 4 | AA, E | TT | 8, 10, 87 | ||
| P05231 | Interleukin - 6 | AA | TT | 8, 46, 87 | ||
| P10145 | Interleukin - 8 | AA | TT | 8, 46, 87 | ||
| P22301 | Interleukin - 10 | AA | M, TT | 8, 46, 87 | ||
| Q9BY25 | IL-1beta | AA | TT | 8, 46, 87 | ||
| P30740 | Leukocyte elastase inhibitor | MS | M, MD | 85 | ||
| P01834 | Ig kappa chain C region | MS | Yes | 85 | ||
| P01842 | Ig lambda chain C regions | MS | Yes | 85 | ||
| P31025 | Lipocalin1 precursor | MS | TT | 85 | ||
| P01871 | Ig mu chain C region | MS | Yes | 85 | ||
| P22079 | Lactoperoxidase | MS | Yes | 85 | ||
| P05164 | Myeloperoxidase | MS | Yes | 85 | ||
| P01833 | Poly-IG receptor protein | MS | TT | 85 | ||
| P12273 | Prolactin-inducible protein | MS | Yes | 85 | ||
| P26447 | S100-A4 | MS | S | 17 | ||
| P05109 | S100-A8 | MS, WB | S, TT | Yes | 18, 85 | |
| P06702 | S100-A9 | MS | S | Yes | 17 | |
| P29508 | Serpin B3 | MS | M, MD | Yes | 14, 85 | |
| P42224 | STAT-1 | MS, AA | S, M | 17, 80 | ||
| P01135 | Transforming growth factor alpha | AA | S | 8, 17 | ||
| P01375 | Tumor necrosis factor alpha | AA | TT | 8, 17 | ||
| Cytoskeleton Related Proteins | ||||||
| P63151 | Actin, cytoplasmic 2 | MS | S, B | 15, 16, 17 | ||
| Q01518 | Adenylyl cyclase | MS | S | 17 | ||
| P12830 | Cadherin-1 | MS, AA | MD, TT | 8, 15, 16, 17 | ||
| P06731 | Carcin-embryonic antigen | MS | Yes | Yes | 85 | |
| P23528 | Cofilin, non-muscle isoform | MS | S, B, M, MD, TT | Yes | 14, 18 | |
| P15311 | Ezrin | MS | S, B | Yes | Yes | 17, 80, 85 |
| P06396 | Gelsolin | MS | S | Yes | Yes | 17, 18, 85 |
| P13646 | Cytokeratin-13 | MS | S | 17 | ||
| P08779 | Cytokeratin-16 | MS | Yes | 85 | ||
| P35527 | Cytokeratin-9 | MS | S, B | Yes | 15 | |
| P35908 | Keratin, type II | MS | S | 17 | ||
| P04264 | Cytokeratin 1 | MS | S, M, MD | Yes | Yes | 17 |
| P19013 | Cytokeratin 4 | MS | Yes | 85 | ||
| P13647 | Cytokeratin-5 | MS | S | 28 | ||
| P02538 | Cytokeratin-6A | MS | Yes | 80 | ||
| P13645 | Cytokeratin-10 | MS | S | 17, 87 | ||
| P02533 | Cytokeratin-14 | MS | TT | Yes | 85 | |
| P19012 | Cytokeratin-15 | MS | M, MD, TT | Yes | 85 | |
| Q04695 | Cytokeratin-17 | MS | TT | 45 | ||
| P08727 | Cytokeratin-19 | MS | S, TT | Yes | 17, 87 | |
| P08729 | Cytokeratin-7 | MS | S, TT | 17, 80, 85 | ||
| P26038 | Moesin | MS | M, MD | Yes | Yes | 80 |
| P07737 | Profilin-1 | MS, WB, E | S, B, M, MD, T | Yes | Yes | 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 85 |
| P03749 | Rho | AA | S | 8, 15, 17 | ||
| P31949 | S100-A11 | MS | TT | Yes | Yes | 85 |
| P35321 | Cornifin-A | MS | Yes | Yes | 85 | |
| P08670 | Vimentin | MS | S, B, M, MD | Yes | 15, 16, 17 | |
| Metabolism Related Proteins | ||||||
| P07108 | AcylCoA binding protein | AA | B | Yes | 15, 16 | |
| Q9DCT1 | Aldo-keto reductase | MS | TT | 14 | ||
| P06733 | Alpha-enolase | MS, WB, E | S, B, M, MD, TT | Yes | Yes | 14, 15, 16, 17, 18, 85 |
| P03973 | Antileukoproteinase 1 precursor | MS | Yes | 85 | ||
| P02647 | Apolipoprotein A-I | MS | S, TT | Yes | Yes | 15, 17 |
| P02649 | Apolipoprotein -E | AA | Yes | 8, 15, 85 | ||
| P06576 | ATP synthase subunit beta | MS | M, MD | 80 | ||
| P00915 | Carbonic anhydrase 1 | MS | TT | Yes | 14, 18 | |
| P23280 | Carbonic anhydrase 6 | MS | Yes | 85 | ||
| P07339 | Cathepsin D | MS, AA, E | M, MD, T, S | 8, 14, 17 | ||
| P04080 | Cystatin B | MS | S | Yes | 17, 85 | |
| P01034 | Cystatin C | MS | B | 16 | ||
| P28325 | Cystatin-D | MS | Yes | 85 | ||
| P01036 | Cystatin-S precursor | MS | Yes | 85 | ||
| P09228 | Cystatin-SA precursor | MS | Yes | 85 | ||
| P01037 | Cystatin-SN precursor | MS | Yes | 85 | ||
| Q01469 | Fatty acid binding protein | MS | S, TT | Yes | Yes | 8, 18, 85 |
| P06744 | Glucose-6-phosphate isomerase | MS | S, M, MD | Yes | 14, 17 | |
| P09211 | Glutathione S-transferase P | MS | S, B, M, MD, TT | 14, 15, 16, 17, 18 | ||
| P04075 | Fructose biphosphate aldolase | MS | M, MD | 14 | ||
| P40926 | Malate dehydrogenase | MS | S, M, MD, T | 17 | ||
| Q06830 | Peroxiredoxin-1 | MS | S, B, TT | Yes | 15, 16, 17, 85 | |
| P30041 | Peroxiredoxin-6 | MS | B, M, MD, TT | 14, 15, 16, | ||
| P00558 | Phosphoglycerate kinase 1 | MS | S | Yes | Yes | 17, 85 |
| P14618 | Pyruvate kinase PKM2 | AA | B, M, MD | Yes | 14, 15, 16, 18, 85 | |
| P10599 | Thioredoxin | MS | S, B, TT | Yes | Yes | 15, 16, 17, 85 |
| P37837 | Transaldolase | MS | S | 17 | ||
| P29401 | TranSetolase | MS | S | 17 | ||
| P60174 | Triosephosphate isomerase | MS | B, M | Yes | 14, 15, 16 | |
| P25311 | Zinc-alpha-2-glycoprotein | MS, 2DMS, E | S, MC | Yes | 17 | |
| Q96DA0 | Zymogen granule protein | MS | Yes | 85 | ||
| Membrane Related Proteins | ||||||
| P04083 | Annexin A1 | AA | S, B, M, MD, TT | Yes | Yes | 15, 16, 17, 74, 85 |
| P46193 | Annexin I | MS, WB | S, B, M, MD | 15, 16, 17 | ||
| P07355 | Annexin II | MS, WB | S, B, M, MD, TT | Yes | Yes | 14, 18, 74, 85 |
| P12429 | Annexin A3 | MS | Yes | 14, 85 | ||
| P15154 | p21 Rac-1 | AA | S | 8, 17 | ||
| P11233 | Ras-related protein Ral-A | AA | TT | 8, 18 | ||
| Calcium Binding Related Proteins | ||||||
| P62158 | Calmodulin | MS | B, M, MD | Yes | Yes | 15, 16, 74, 85 |
| P26447 | S100-A4 | MS | S, B | 15, 16, 17 | ||
| P06703 | S100-A6 | MS | S | Yes | Yes | 17, 74, 85 |
| P31151 | S100-A7 | MS | S, TT | 16, 17 | ||
| Anti-Microbial Related Proteins | ||||||
| P59666 | Neutrophil defensin 3 | MS | Yes | 85, 87 | ||
| P15515 | Histatin-1 precursor | MS | Yes | 85 | ||
| Q9HC84 | Mucin-5B precursor | MS | Yes | 85 | ||
| Q8TAX7 | Mucin-7 | MS | Yes | 85 | ||
| P02814 | Submax. gland androgen-reg. prot. | MS, 2DMS, E | S, M, MB | 17 | ||
| P02788 | Lactotransferrin | MS | Yes | 85 | ||
| P15941 | Cancer antigen 15-3 | E | Yes | Yes | 29, 85 | |
Abbreviations: The UniProt identification number and protein name are from UniProt database (www.uniprot.org). Method of Identification: MS = Mass spectrometry, 2DMS = 2D-Gel Spot Mass Spectrometry, E = ELISA, WB = Western blot, AA = Antibody Array. Cell Lines: S = SKBR3, M = MCF7, T = T47D, MD = MB-MDA-231, B = 8701-BC, TT = Tumor Tissue. Ref. = References.