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. 2016 Aug 1;6:30640. doi: 10.1038/srep30640

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Copyright © 2016, The Author(s)

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The effects of blebbistatin on ring contraction after 1 h (black) and viability (red) in ASMC (a) and A10 (c) rings showed an expected result, in which ASMC and A10 rings were unable to contract as quickly as vehicle-treated rings with higher concentrations of blebbistatin, without any toxic effect (n = 3 rings for 8 concentrations). Immunofluorescent stains of ASMC (b) and A10 (d) rings for pMLC (red) of those rings after 5 min of contraction, with nuclei counterstained with DAPI (blue), showed that with higher amounts of blebbistatin, which binds myosin light chain, the rings stain less intensely for pMLC, in keeping with the slower rate of contraction. 100% = maximum, 0% = minimum. Scale bar = 50 μm. *p < 0.05 v. control.