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. 2016 Aug 1;6:30640. doi: 10.1038/srep30640

Figure 3. Effects of norepinephrine on vascular smooth muscle ring contraction.

Figure 3

Norepinphrine had an expected effect on ring contraction (black) and viability (red) in ASMC (a, contraction after 1.5 h) and A10 (c, contraction over 4 h), where ASMC and A10 rings contracted faster than controls without any toxic effect (n = 3 rings for 8 concentrations). Immunofluorescent stains of ASMC (b) and A10 (d) rings for pMLC (red) of those rings after 5 min of contraction, with nuclei counterstained with DAPI (blue), showed that the stain intensity for pMLC increased in ASMC rings with higher concentrations of norepinephrine, but decreased in A10 rings. 100% = maximum, 0% = minimum. Scale bar = 50 μm. *p < 0.05 v. control.